Validation Data Gallery
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Tested Applications
| Positive WB detected in | Daudi cells, Raji cells, Ramos cells, human plasma |
| Positive IF/ICC detected in | Ramos cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86939-1-RR targets IGJ in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag4559 Product name: Recombinant human IGJ protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-159 aa of BC038982 Sequence: MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD 相同性解析による交差性が予測される生物種 |
| Full Name | immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides |
| Calculated molecular weight | 158 aa, 18 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC038982 |
| Gene Symbol | IGJ |
| Gene ID (NCBI) | 3512 |
| RRID | AB_3745221 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01591 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IGJ antibody 86939-1-RR | Download protocol |
| WB protocol for IGJ antibody 86939-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |