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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IGJ Recombinant monoclonal antibody (86939-1-RR)

    WB analysis using 86939-1-RR (same clone as 86939-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86939-1-RR (IGJ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86939-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Ramos cells using IGJ Recombinant monoclonal antibody (86939-1-RR)

    IF Staining of Ramos using 86939-1-RR (same clone as 86939-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using IGJ antibody (86939-1-RR, Clone: 251691G5 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86939-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86939-1-RR

    Affinity and Kinetic Characterization of 86939-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86939-1-RR against Human IGJ were performed. The affinity constant is below 1 pM.

IGJ Recombinant monoclonal antibody, PBS Only

IGJ Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86939-1-PBS
Clone No.251691G5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

IGCJ, Immunoglobulin J chain, JCH, JCHAIN, Joining chain of multimeric IgA and IgM

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86939-1-PBS targets IGJ in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag4559

Product name: Recombinant human IGJ protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-159 aa of BC038982

Sequence: MKNHLLFWGVLAVFIKAVHVKAQEDERIVLVDNKCKCARITSRIIRSSEDPNEDIVERNIRIIVPLNNRENISDPTSPLRTRFVYHLSDLCKKCDPTEVELDNQIVTATQSNICDEDSATETCYTYDRNKCYTAVVPLVYGGETKMVETALTPDACYPD

相同性解析による交差性が予測される生物種
Full Name immunoglobulin J polypeptide, linker protein for immunoglobulin alpha and mu polypeptides
Calculated molecular weight 158 aa, 18 kDa
Observed molecular weight25 kDa
GenBank accession numberBC038982
Gene Symbol IGJ
Gene ID (NCBI) 3512
RRIDAB_3745221
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01591
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

IGJ, the immunoglobulin J chain, serves to link two monomer units of either IgM or IgA.

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