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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFT172 Polyclonal antibody (28441-1-AP)

    WB analysis using 28441-1-AP (same clone as 28441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28441-1-AP (IFT172 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28441-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IFT172 Polyclonal antibody (28441-1-AP)

    WB analysis using 28441-1-AP (same clone as 28441-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28441-1-AP (IFT172 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IFT172 Polyclonal antibody (28441-1-AP)

    IHC staining of mouse testis using 28441-1-AP (same clone as 28441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28441-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IFT172 Polyclonal antibody (28441-1-AP)

    IHC staining of mouse testis using 28441-1-AP (same clone as 28441-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28441-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using IFT172 Polyclonal antibody (28441-1-AP)

    IF Staining of ARPE-19 using 28441-1-AP (same clone as 28441-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using IFT172 antibody (28441-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28441-1-PBS in a different storage buffer formulation.

IFT172 Polyclonal antibody, PBS Only

IFT172 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28441-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28441-1-PBS targets IFT172 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28921

Product name: Recombinant human IFT172 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1206-1408 aa of BC137126

Sequence: QARGALEEKDFQKAEGLLLRAQRPGLALNYYKEAGLWSDALRICKDYVPSQLEALQEEYEREATKKGARGVEGFVEQARHWEQAGEYSRAVDCYLKVRDSGNSGLAEKCWMKAAELSIKFLPPQRNMEVVLAVGPQLIGIGKHSAAAELYLNLDLVKEAIDAFIEGEEWNKAKRVAKELDPRYEDYVDQHYKEFLKNQGKVDS

相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 172 homolog (Chlamydomonas)
Calculated molecular weight 1749 aa, 198 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC137126
Gene Symbol IFT172
Gene ID (NCBI) 26160
RRIDAB_2881142
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UG01
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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