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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IFT172 Polyclonal antibody

IFT172 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28441-1-AP

Synonyms

DKFZp434A163, IFT172, KIAA1179, osm 1, SLB, wim

Validation Data Gallery

Applications:
  • WB analysis using 28441-1-AP

    WB analysis using 28441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28441-1-AP (IFT172 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 28441-1-AP

    WB analysis using 28441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28441-1-AP (IFT172 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 28441-1-AP

    IHC staining of mouse testis using 28441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 28441-1-AP

    IHC staining of mouse testis using 28441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue, mouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28441-1-AP targets IFT172 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT172 fusion protein Ag28921 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 172 homolog (Chlamydomonas)
Calculated molecular weight 1749 aa, 198 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC137126
Gene symbol IFT172
Gene ID (NCBI) 26160
RRIDAB_2881142
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFT172 antibody 28441-1-APDownload protocol
IHC protocol for IFT172 antibody 28441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols