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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFT172 Polyclonal antibody (28441-1-AP)

    WB analysis using 28441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28441-1-AP (IFT172 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IFT172 Polyclonal antibody (28441-1-AP)

    WB analysis using 28441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28441-1-AP (IFT172 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IFT172 Polyclonal antibody (28441-1-AP)

    IHC staining of mouse testis using 28441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using IFT172 Polyclonal antibody (28441-1-AP)

    IHC staining of mouse testis using 28441-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 28441-1-AP (IFT172 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of ARPE-19 cells using IFT172 Polyclonal antibody (28441-1-AP)

    IF Staining of ARPE-19 using 28441-1-AP

    Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using IFT172 antibody (28441-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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IFT172 Polyclonal antibody

IFT172 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28441-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp434A163, IFT172, KIAA1179, osm 1, SLB, wim

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue, mouse brain tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inARPE-19 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28441-1-AP targets IFT172 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Reactivity mouse, rat, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT172 fusion protein Ag28921 相同性解析による交差性が予測される生物種
Full Name intraflagellar transport 172 homolog (Chlamydomonas)
Calculated molecular weight 1749 aa, 198 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC137126
Gene Symbol IFT172
Gene ID (NCBI) 26160
RRIDAB_2881142
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UG01
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IFT172 antibody 28441-1-APDownload protocol
IHC protocol for IFT172 antibody 28441-1-APDownload protocol
IF protocol for IFT172 antibody 28441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols