Validation Data Gallery
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Tested Applications
| Positive WB detected in | Recombinant protein, Transfected with Human IFN alpha 2B CHO cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85261-2-RR targets IFN alpha 2B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Product name: HumanKine® recombinant human IFN alpha 2B protein Source: -derived, Tag: Sequence: 相同性解析による交差性が予測される生物種 |
| Full Name | interferon, alpha 2 |
| Gene Symbol | IFN alpha2 |
| Gene ID (NCBI) | 3440 |
| RRID | AB_3744126 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P01563 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interferon alpha 2 is a type 1 interferon. Interferon alpha 2 is secreted by virus-infected cells and inhibits other cells from further infection. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFN alpha 2B antibody 85261-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |