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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IFN alpha 2B Recombinant monoclonal antibody (85261-2-RR)

    WB analysis using 85261-2-RR (same clone as 85261-2-PBS)

    Human IFN alpha 2 Recombinant protein was subjected to SDS PAGE followed by western blot with 85261-2-RR (IFN alpha 2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85261-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IFN alpha 2B Recombinant monoclonal antibody (85261-2-RR)

    WB analysis using 85261-2-RR (same clone as 85261-2-PBS)

    Human IFN alpha 2 B-transfected CHO cell lysate was subjected to SDS PAGE followed by western blot with 85261-2-RR (IFN alpha 2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85261-2-PBS in a different storage buffer formulation.

IFN alpha 2B Recombinant monoclonal antibody, PBS Only

IFN alpha 2B Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 85261-2-PBS
Clone No.250407B7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

IFN alpha 2, IFNA2, IFN alpha 2A, IFN alpha2, IFN-alpha 2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85261-2-PBS targets IFN alpha 2B in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo:

Product name: HumanKine® recombinant human IFN alpha 2B protein

Source: -derived,

Tag:

Domain: of

Sequence:

相同性解析による交差性が予測される生物種
Full Name interferon, alpha 2
Gene Symbol IFN alpha2
Gene ID (NCBI) 3440
RRIDAB_3744126
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP01563
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Interferon alpha 2 is a type 1 interferon. Interferon alpha 2 is secreted by virus-infected cells and inhibits other cells from further infection. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761)

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