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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated IFIT3 Monoclonal antibody

IFIT3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2C8B10

Cat no : CL594-67208

Synonyms

CIG 49, CIG49, GARG 49, IFI 60K, IFI60, IFIT 3, IFIT 4, IFIT3, IFIT4, IRG2, ISG 60, ISG60, RIG G

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-67208

    IF Staining of HepG2 using CL594-67208

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594-conjugated IFIT3 antibody (CL594-67208, Clone: 2C8B10 ) at dilution of 1:100.

Tested Applications

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67208-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-67208 targets IFIT3 in IF applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IFIT3 fusion protein Ag7518 相同性解析による交差性が予測される生物種
Full Name IFIT3
Calculated molecular weight 56 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC004977
Gene symbol IFIT3
Gene ID (NCBI) 3437
RRIDAB_2920096
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

IFIT3 (IFN-induced protein with tetratricopeptide repeats 3), also known as IFIT-4, ISG-60 or RIG-G, is a 56 kDa IFN induced protein. IFIT3 has been identified to be associated with strong IFN antiviral activity. Upon RNA virus infection, IFIT3 is significantly induced, ectopic expression or knockdown of IFIT3 could, respectively, enhance or impair IFN regulatory factor 3 (IRF3)-mediated gene expression. IFIT3 thus may serve as an important modulator in innate immunity, revealing a new function of the IFIT family proteins.

Protocols

Product Specific Protocols
IF protocol for CL594 IFIT3 antibody CL594-67208Download protocol
Standard Protocols
Click here to view our Standard Protocols