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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IER3IP1 Polyclonal antibody (29402-1-AP)

    WB analysis using 29402-1-AP (same clone as 29402-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29402-1-AP (IER3IP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29402-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IER3IP1 Polyclonal antibody (29402-1-AP)

    IHC staining of human kidney using 29402-1-AP (same clone as 29402-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29402-1-AP (IER3IP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29402-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using IER3IP1 Polyclonal antibody (29402-1-AP)

    IF Staining of U2OS using 29402-1-AP (same clone as 29402-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IER3IP1 antibody (29402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29402-1-PBS in a different storage buffer formulation.

IER3IP1 Polyclonal antibody, PBS Only

IER3IP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29402-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29402-1-PBS targets IER3IP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag28027

Product name: Recombinant human IER3IP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-82 aa of BC010888

Sequence: MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVRTVMRVPLIIVNSIAIVLLLLFG

相同性解析による交差性が予測される生物種
Full Name immediate early response 3 interacting protein 1
Calculated molecular weight 82 aa, 9 kDa
Observed molecular weight9 kDa
GenBank accession numberBC010888
Gene Symbol IER3IP1
Gene ID (NCBI) 51124
RRIDAB_2918295
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5U9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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