Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29402-1-AP targets IER3IP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28027 Product name: Recombinant human IER3IP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-82 aa of BC010888 Sequence: MAFTLYSLLQAALLCVNAIAVLHEERFLKNIGWGTDQGIGGFGEEPGIKSQLMNLIRSVRTVMRVPLIIVNSIAIVLLLLFG 相同性解析による交差性が予測される生物種 |
| Full Name | immediate early response 3 interacting protein 1 |
| Calculated molecular weight | 82 aa, 9 kDa |
| Observed molecular weight | 9 kDa |
| GenBank accession number | BC010888 |
| Gene Symbol | IER3IP1 |
| Gene ID (NCBI) | 51124 |
| RRID | AB_2918295 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5U9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for IER3IP1 antibody 29402-1-AP | Download protocol |
| IHC protocol for IER3IP1 antibody 29402-1-AP | Download protocol |
| WB protocol for IER3IP1 antibody 29402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |