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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH3B Polyclonal antibody (15576-1-AP)

    WB analysis using 15576-1-AP (same clone as 15576-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15576-1-AP (IDH3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IDH3B Polyclonal antibody (15576-1-AP)

    IHC staining of mouse brain using 15576-1-AP (same clone as 15576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15576-1-AP (IDH3B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IDH3B Polyclonal antibody (15576-1-AP)

    IHC staining of mouse brain using 15576-1-AP (same clone as 15576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15576-1-AP (IDH3B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using IDH3B Polyclonal antibody (15576-1-AP)

    IF Staining of A549 using 15576-1-AP (same clone as 15576-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using IDH3B antibody (15576-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IDH3B Polyclonal antibody (15576-1-AP)

    IF Staining of HepG2 using 15576-1-AP (same clone as 15576-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IDH3B antibody (15576-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

IDH3B Polyclonal antibody, PBS Only

IDH3B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15576-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

H IDHB, NAD(+)-specific ICDH subunit beta, RP46

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15576-1-PBS targets IDH3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7965

Product name: Recombinant human IDH3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-385 aa of BC001960

Sequence: MAALSGVRWLTRALVSAGNPGAWRGLSTSAAAHAASRSQAEDVRVEGSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQTKGS

相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 3 (NAD+) beta
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC001960
Gene Symbol IDH3B
Gene ID (NCBI) 3420
RRIDAB_3741868
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43837
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).

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