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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH3B Polyclonal antibody (15576-1-AP)

    WB analysis using 15576-1-AP (same clone as 15576-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15576-1-AP (IDH3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IDH3B Polyclonal antibody (15576-1-AP)

    IHC staining of mouse brain using 15576-1-AP (same clone as 15576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15576-1-AP (IDH3B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IDH3B Polyclonal antibody (15576-1-AP)

    IHC staining of mouse brain using 15576-1-AP (same clone as 15576-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15576-1-AP (IDH3B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using IDH3B Polyclonal antibody (15576-1-AP)

    IF Staining of A549 using 15576-1-AP (same clone as 15576-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using IDH3B antibody (15576-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using IDH3B Polyclonal antibody (15576-1-AP)

    IF Staining of HepG2 using 15576-1-AP (same clone as 15576-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IDH3B antibody (15576-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15576-1-PBS in a different storage buffer formulation.

IDH3B Polyclonal antibody, PBS Only

IDH3B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15576-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

H IDHB, NAD(+)-specific ICDH subunit beta, RP46

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15576-1-PBS targets IDH3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7965

Product name: Recombinant human IDH3B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-385 aa of BC001960

Sequence: MAALSGVRWLTRALVSAGNPGAWRGLSTSAAAHAASRSQAEDVRVEGSFPVTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVAIIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGEYSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFLQCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGVVPGESYSAEYAVFETGARHPFAQAVGRNIANPTAMLLSASNMLRHLNLEYHSSMIADAVKKVIKVGKVRTRDMGGYSTTTDFIKSVIGHLQTKGS

相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 3 (NAD+) beta
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC001960
Gene Symbol IDH3B
Gene ID (NCBI) 3420
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43837
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).

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