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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IDH3B Polyclonal antibody (15576-1-AP)

    WB analysis using 15576-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15576-1-AP (IDH3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IDH3B Polyclonal antibody (15576-1-AP)

    IHC staining of mouse brain using 15576-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15576-1-AP (IDH3B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using IDH3B Polyclonal antibody (15576-1-AP)

    IHC staining of mouse brain using 15576-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15576-1-AP (IDH3B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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IDH3B Polyclonal antibody

IDH3B Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15576-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

H IDHB, NAD(+)-specific ICDH subunit beta, RP46

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, Jurkat cells, LNCaP cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15576-1-AP targets IDH3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IDH3B fusion protein Ag7965 相同性解析による交差性が予測される生物種
Full Name isocitrate dehydrogenase 3 (NAD+) beta
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC001960
Gene Symbol IDH3B
Gene ID (NCBI) 3420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).

Protocols

Product Specific Protocols
WB protocol for IDH3B antibody 15576-1-APDownload protocol
IHC protocol for IDH3B antibody 15576-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols