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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ICAM4 Monoclonal antibody (67014-1-Ig)

    WB analysis using 67014-1-Ig (same clone as 67014-1-PBS)

    Different amount of human blood lysates were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human red blood cells using ICAM4 Monoclonal antibody (67014-1-Ig)

    WB analysis of human red blood cells using 67014-1-Ig (same clone as 67014-1-PBS)

    human red blood cells were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human spleen tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human placenta using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human placenta using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human colon using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IHC staining of human colon using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse spleen tissue using ICAM4 Monoclonal antibody (67014-1-Ig)

    IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

ICAM4 Monoclonal antibody, PBS Only

ICAM4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 67014-1-PBS
Clone No.3A9B9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CD242, ICAM 4, ICAM-4, Landsteiner-Wiener blood group glycoprotein, LW

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67014-1-PBS targets ICAM4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag27628

Product name: Recombinant human ICAM4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 23-152 aa of BC029364

Sequence: ALGRRTKRAQSPKGSPLAPSGTSVPFWVRMSPEFVAVQPGKSVQLNCSNSCPQPQNSSLRTPLRQGKTLRGPGWVSYQLLDVRAWSSLAHCLVTCAGKTRWATSRITAYKPPHSVILEPPVLKGRKYTLR

相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
Calculated molecular weight 271 aa, 29 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC029364
Gene Symbol ICAM4
Gene ID (NCBI) 3386
RRIDAB_2882330
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ14773
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

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