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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

ICAM4 Monoclonal antibody

ICAM4 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3A9B9

Cat no : 67014-1-Ig

Synonyms

CD242, ICAM 4, ICAM4, ICAM-4, LW, LW blood group protein

Validation Data Gallery

Applications:
  • WB analysis using 67014-1-Ig

    WB analysis using 67014-1-Ig

    Different amount of human blood lysates were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • WB analysis of human red blood cells using 67014-1-Ig

    WB analysis of human red blood cells using 67014-1-Ig

    human red blood cells were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67014-1-Ig

    IHC staining of human spleen using 67014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IHC staining of human spleen using 67014-1-Ig

    IHC staining of human spleen using 67014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IHC staining of human colon using 67014-1-Ig

    IHC staining of human colon using 67014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IHC staining of human colon using 67014-1-Ig

    IHC staining of human colon using 67014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IHC staining of human placenta using 67014-1-Ig

    IHC staining of human placenta using 67014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IHC staining of human placenta using 67014-1-Ig

    IHC staining of human placenta using 67014-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IF Staining of mouse spleen using 67014-1-Ig

    IF Staining of mouse spleen using 67014-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of mouse spleen using 67014-1-Ig

    IF Staining of mouse spleen using 67014-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman blood tissue, human red blood cells
Positive IHC detected inhuman spleen tissue, human colon tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse spleen tissue
Planning an IHC experiment? We recommend our IHCeasy ICAM4 Ready-To-Use IHC Kit. ICAM4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

67014-1-Ig targets ICAM4 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen ICAM4 fusion protein Ag27628 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
Calculated molecular weight 271 aa, 29 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC029364
Gene symbol ICAM4
Gene ID (NCBI) 3386
RRIDAB_2882330
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Protocols

Product Specific Protocols
WB protocol for ICAM4 antibody 67014-1-IgDownload protocol
IHC protocol for ICAM4 antibody 67014-1-IgDownload protocol
IF protocol for ICAM4 antibody 67014-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Clin Endocrinol Metab

LncRNA LPAL2/miR-1287-5p/EGFR axis modulates TED derived orbital fibroblast activation through cell adhesion factors.

Authors - Nuo Wang
View Article