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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    WB analysis of Jurkat using 17699-1-AP (same clone as 17699-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    WB analysis of Jurkat using 17699-1-AP (same clone as 17699-1-PBS)

    WB result of ICAM3/CD50 antibody (17699-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ICAM3/CD50 transfected Jurkat cells. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Ramos cells using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    WB analysis of Ramos using 17699-1-AP (same clone as 17699-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    WB analysis of U-937 using 17699-1-AP (same clone as 17699-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Jurkat cells using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    IP experiment of Jurkat using 17699-1-AP (same clone as 17699-1-PBS)

    IP result of anti-ICAM3/CD50 (IP:17699-1-AP, 4ug; Detection:17699-1-AP 1:500) with Jurkat cells lysate 4000 ug. This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    IHC staining of human colon using 17699-1-AP (same clone as 17699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    IHC staining of human colon using 17699-1-AP (same clone as 17699-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17699-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ICAM3/CD50 Polyclonal antibody, PBS Only

ICAM3/CD50 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17699-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

CDW50, ICAM3, CD50, ICAM 3, ICAM R

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17699-1-PBS targets ICAM3/CD50 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11955

Product name: Recombinant human ICAM3 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 200-547 aa of BC058903

Sequence: GLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSHFVPVFVAVLLTLGVVTIVLALMYVFREHQRSGSYHVREESTYLPLTSMQPTEAMGEEPSRAE

相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 3
Calculated molecular weight 547 aa, 60 kDa
Observed molecular weight 110-160 kDa, 60 kDa
GenBank accession numberBC058903
Gene Symbol ICAM3/CD50
Gene ID (NCBI) 3385
RRIDAB_2248763
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP32942
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ICAM3 (Intercellular adhesion molecule 3) is also named as CD50, CDw50 and ICAM-R. ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. ICAM3, being expressed constitutively on T cells and other leukocytes, is a potent signalling molecule and a major ligand in the initiation of T cell-mediated immune responses (PMID: 1448173, PMID: 11812993). ICAM3 is the natural ligand for both DC-SIGN and L-SIGN (PMID: 17570115). ICAM3 is abundantly and constitutively expressed in all leukocytes and is probably the most important ligand for LFA-1 in initiating immune responses, and ICAM3 is a typical type I transmembrane glycoprotein (PMID: 39991572). The promoter region of ICAM3 can be bound by co-transcription factors such as ETs and C/EBP, promoting macrophage maturation as well as monocyte extravasation (PMID: 22479382). ICAM3 is widely expressed not only in lymphocytes such as T and B cells but also in many endothelial cells and dendritic cells (PMID: 39991572).

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