• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Jurkat cells using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    WB analysis of Jurkat using 17699-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using ICAM3 Polyclonal antibody (17699-1-AP)

    WB analysis of U-937 using 17699-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using ICAM3 Polyclonal antibody (17699-1-AP)

    WB analysis of Ramos using 17699-1-AP

    Ramos cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    IP experiment of Jurkat using 17699-1-AP

    IP result of anti-ICAM3/CD50 (IP:17699-1-AP, 4ug; Detection:17699-1-AP 1:500) with Jurkat cells lysate 4000 ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using ICAM3/CD50 Polyclonal antibody (17699-1-AP)

    IHC staining of human colon using 17699-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ICAM3 Polyclonal antibody (17699-1-AP)

    IHC staining of human colon using 17699-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ICAM3/CD50 Polyclonal antibody

ICAM3/CD50 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 17699-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

CDW50, ICAM3, CD50, ICAM 3, ICAM R

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, Ramos cells, U-937 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17699-1-AP targets ICAM3/CD50 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICAM3/CD50 fusion protein Ag11955 相同性解析による交差性が予測される生物種
Full Name intercellular adhesion molecule 3
Calculated molecular weight 547 aa, 60 kDa
Observed molecular weight 110-160 kDa, 60 kDa
GenBank accession numberBC058903
Gene Symbol ICAM3/CD50
Gene ID (NCBI) 3385
RRIDAB_2248763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ICAM3 (Intercellular adhesion molecule 3) is also named as CD50, CDw50 and ICAM-R. ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. ICAM3, being expressed constitutively on T cells and other leukocytes, is a potent signalling molecule and a major ligand in the initiation of T cell-mediated immune responses (PMID: 1448173, PMID: 11812993). ICAM3 is the natural ligand for both DC-SIGN and L-SIGN (PMID: 17570115). ICAM3 is abundantly and constitutively expressed in all leukocytes and is probably the most important ligand for LFA-1 in initiating immune responses, and ICAM3 is a typical type I transmembrane glycoprotein (PMID: 39991572). The promoter region of ICAM3 can be bound by co-transcription factors such as ETs and C/EBP, promoting macrophage maturation as well as monocyte extravasation (PMID: 22479382). ICAM3 is widely expressed not only in lymphocytes such as T and B cells but also in many endothelial cells and dendritic cells (PMID: 39991572).

Protocols

Product Specific Protocols
WB protocol for ICAM3/CD50 antibody 17699-1-APDownload protocol
IHC protocol for ICAM3/CD50 antibody 17699-1-APDownload protocol
IP protocol for ICAM3/CD50 antibody 17699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols