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CoraLite®594-conjugated HuR/ELAVL1 Polyclonal antibody
HuR/ELAVL1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-11910
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive FC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy HuR/ELAVL1 Ready-To-Use IHC Kit. HuR/ELAVL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11910-1-AP
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL594-11910 targets HuR/ELAVL1 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | HuR/ELAVL1 fusion protein Ag2249 相同性解析による交差性が予測される生物種 |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) |
| Calculated molecular weight | 326 aa, 36 kDa |
| Observed molecular weight | 30-37 kDa |
| GenBank accession number | BC003376 |
| Gene symbol | ELAVL1 |
| Gene ID (NCBI) | 1994 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 HuR/ELAVL1 antibody CL594-11910 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
