Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | PC-12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-24992 targets HuD-specific in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21830 Product name: Recombinant human ELAVL4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-40 aa of BC036071 Sequence: MVMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGAT 相同性解析による交差性が予測される生物種 |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D) |
| Calculated molecular weight | 380 aa, 42 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | BC036071 |
| Gene Symbol | ELAVL4 |
| Gene ID (NCBI) | 1996 |
| RRID | AB_3084087 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26378 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ELAVL4, also named as HuD, is a 380 amino acid protein, which belongs to the RRM elav family. ELAVL4 is expressed in brain and localizes in the nucleus. ELAVL4 may play a role in neuron-specific RNA processing and protects CDKN1A mRNA from decay by binding to its 3'-UTR. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 HuD-specific antibody CL488-24992 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |