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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Histone H2B Polyclonal antibody (15857-1-AP)

    WB analysis using 15857-1-AP (same clone as 15857-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using Histone H2B Polyclonal antibody (15857-1-AP)

    WB analysis using 15857-1-AP (same clone as 15857-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    WB analysis of mouse pancreas using 15857-1-AP (same clone as 15857-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat kidney tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    WB analysis of rat kidney using 15857-1-AP (same clone as 15857-1-PBS)

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using Histone H2B Polyclonal antibody (15857-1-AP)

    IP experiment of HEK-293 using 15857-1-AP (same clone as 15857-1-PBS)

    IP result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    IHC staining of human prostate cancer using 15857-1-AP (same clone as 15857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    IHC staining of human breast cancer using 15857-1-AP (same clone as 15857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Histone H2B Polyclonal antibody (15857-1-AP)

    IHC staining of human breast cancer using 15857-1-AP (same clone as 15857-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining using Histone H2B Polyclonal antibody (15857-1-AP)

    IF Staining using 15857-1-AP (same clone as 15857-1-PBS)

    Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School. This data was developed using the same antibody clone with 15857-1-PBS in a different storage buffer formulation.

Histone H2B Polyclonal antibody, PBS Only

Histone H2B Polyclonal Antibody for WB, IHC, IF, IP, Indirect ELISA
Cat No. 15857-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, Indirect ELISA

Synonyms

HIST2H2BE, GL105, H2B, H2B.1, H2B/q

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15857-1-PBS targets Histone H2B in WB, IHC, IF, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag7811

Product name: Recombinant human Histone H2B protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-126 aa of BC005827

Sequence: MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK

相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H2be
Calculated molecular weight 14 kDa
Observed molecular weight14-17 kDa
GenBank accession numberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
RRIDAB_10664929
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16778
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Histone H2B is a core component of the nucleosome, the fundamental unit of chromatin. It forms a dimer with Histone H2A, and two H2A-H2B dimers combine with a (H3-H4)₂ tetramer to create the histone octamer. The primary function of this structure is to package DNA into a compact and manageable form. However, H2B is also subject to various post-translational modifications (PTMs), such as ubiquitination, which serve as epigenetic marks to regulate gene expression by altering chromatin accessibility.

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