Validation Data Gallery

Filter: AllWBIPIHCIF

WB analysis using 15857-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 15857-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 15857-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat kidney using 15857-1-AP
rat kidney tissue were subjected to SDS PAGE followed by western blot with 15857-1-AP (Histone H2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 15857-1-AP
IP result of anti-Histone H2B (IP:15857-1-AP, 4ug; Detection:15857-1-AP 1:500) with HEK-293 cells lysate 2000ug.
IHC staining of human prostate cancer using 15857-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 15857-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15857-1-AP (Histone H2B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 15857-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human breast cancer using 15857-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15857-1-AP (Histone H2B Antibody) at dilution of 1:50 (under 10x lens).
IF Staining using 15857-1-AP
Mouse tumour sections were labelled with H2B (15857-1-AP, red), Dendra2 (green), and DAPI. The image was credited by Dr. Hae Lin Jang from Harvard Medical School.

Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Cat No. 15857-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse pancreas tissue, Jurkat cells, rat kidney tissue, HeLa cells, U2OS cells, mouse kidney tissue
Positive IP detected in	HEK-293 cells
Positive IHC detected in	human prostate cancer tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	N/A

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)	IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 12 publications below
IF	See 5 publications below

Product Information

15857-1-AP targets Histone H2B in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, bovine
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Histone H2B fusion protein Ag7811 相同性解析による交差性が予測される生物種
Full Name	histone cluster 2, H2be
Calculated molecular weight	14 kDa
Observed molecular weight	14-17 kDa
GenBank accession number	BC005827
Gene Symbol	Histone H2B
Gene ID (NCBI)	8349
RRID	AB_10664929
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16778
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for Histone H2B antibody 15857-1-AP	Download protocol
IHC protocol for Histone H2B antibody 15857-1-AP	Download protocol
IP protocol for Histone H2B antibody 15857-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Nanotechnol Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells. Authors - Tanmoy Saha View Article
human	IF	Nat Biomed Eng Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues Authors - Su-Jin Heo View Article
human	WB	Oncogene HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis. Authors - Laura Monteonofrio View Article
human	WB	Elife Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia. Authors - William F Richter View Article
human	WB	Mol Cell Proteomics Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells. Authors - Rui Sun View Article
human	WB	Cell Discov USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment. Authors - Jialun Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 15857-1-AP

WB analysis using 15857-1-AP

WB analysis of mouse pancreas using 15857-1-AP

WB analysis of rat kidney using 15857-1-AP

IP experiment of HEK-293 using 15857-1-AP

IHC staining of human prostate cancer using 15857-1-AP

IHC staining of human prostate cancer using 15857-1-AP

IHC staining of human breast cancer using 15857-1-AP

IHC staining of human breast cancer using 15857-1-AP

IF Staining using 15857-1-AP

Histone H2B Polyclonal antibody

Histone H2B Polyclonal Antibody for WB, IP, IF, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Intercellular nanotubes mediate mitochondrial trafficking between cancer and immune cells.

Aberrant chromatin reorganization in cells from diseased fibrous connective tissue in response to altered chemomechanical cues

HIPK2 and extrachromosomal histone H2B are separately recruited by Aurora-B for cytokinesis.

Non-canonical H3K79me2-dependent pathways promote the survival of MLL-rearranged leukemia.

Chemoproteomics Reveals Chemical Diversity and Dynamics of 4-Oxo-2-nonenal Modifications in Cells.

USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.