Validation Data Gallery
Product Information
The ChromoTek His Fab-Trap® Magnetic Particles M-270 Kit consists of an anti-His-tag Fab-fragment, which is covalently bound to magnetic particles. It also contains lysis, wash, and elution buffers that can be used for the immunoprecipitation of His-tagged proteins from cell extracts of various organisms. It is highly recommended when very large proteins/complexes are being investigated.
| Description | The His Fab-Trap® Magnetic Agarose Kit contains His Fab-Trap® Magnetic Particles M-270, lysis, wash, and elution buffers for efficient immunoprecipitation of His-tagged proteins. • Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • Low background • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, yeast, etc. |
| Applications | IP, Co-IP |
| Specificity/Target | Binds specifically to the His-tag (also known as hexahistidine or His6-tag) fused to a protein of interest at N-, C- or internal position. Please note that the affinity is highest for a C-terminal fusion (dissociation constant KD of 10 nM for a C-terminal His-tag and 220 nM for an N-terminal His-tag). |
| Binding Capacity | 1 μg of recombinant His-tagged protein (~30 kDa) per 25 μL bead slurry |
| Conjugate | Magnetic Particles M-270; size: 2.8 µM |
| Elution buffer | 2x SDS-sample buffer (Lämmli), 200 mM glycine pH 2.5 or 100 µM His-Peptide |
| Wash Buffer Compatibility | 2 M NaCl, 5 mM DTT, 5 mM β-mercaptoethanol, 5 mM TCEP, 2% NP40, 2% Triton X-100, <0.1% SDS, 3-4 M Urea |
| Type | Fab-fragment |
| Class | Recombinant |
| Host | Mouse |
| Affinity (KD) | 10 nM for a C-terminal His-tag and 220 nM for a N-terminal His-tag |
| Compatibility with mass spectrometry | The His Fab-Trap® is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_3665061 |
| Storage Buffer | PBS pH 7.4. Preservative: 0.09% sodium azide |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at +4°C. Stable for one year. Do not freeze! |
Kit components
| Component | Description |
|---|---|
| His Fab-Trap® Magnetic Particles M-270 | 20 reactions (500 µl) |
| Lysis buffer | Optimized for cytoplasmic proteins and mammalian cell lysis |
| RIPA buffer | Optimized for nuclear/chromatin proteins and mammalian cell lysis |
| Wash buffer | Removal of unwanted proteins, peptides, etc. |
| Dilution buffer | Dilution of cell lysate |
| Elution buffer | For acidic elution |