CoraLite® Plus 488-conjugated Hemopexin Monoclonal antibody

Hemopexin Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3A9D6

Cat no : CL488-66479

Synonyms

Beta 1B glycoprotein, hemopexin, HPX



Tested Applications

Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HPX Ready-To-Use IHC Kit. HPX primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66479-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66479 targets Hemopexin in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Hemopexin fusion protein Ag8533 相同性解析による交差性が予測される生物種
Full Name hemopexin
Calculated molecular weight 254 aa, 29 kDa
Observed molecular weight 65-75 kDa
GenBank accession numberBC005395
Gene symbol HPX
Gene ID (NCBI) 3263
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hemopexin (HPX) is the plasma protein responsible for scavenging heme, thus preventing heme-mediated oxidative stress and heme-bound iron loss. In addition, hemopexin blocks heme activation of immune receptors and vascular inflammatory processes. It is mainly expressed in liver, the synthesis of which is induced after inflammation. Alterations of plasma hemopexin level have been linked to disorders like atherosclerosis and inflammatory diseases.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Hemopexin antibody CL488-66479Download protocol
Standard Protocols
Click here to view our Standard Protocols