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Validation Data Gallery

  • Halo-Trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. Coomassie and Western blot. Halo-tag antibody [28A8], monoclonal mouse IgG1and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

    Halo-Trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. Coomassie and Western blot. Halo-tag antibody [28A8], monoclonal mouse IgG1and anti-mouse secondary antibody. I: Input, FT: Flow-Through, B: Bound

  • Halo-trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. I: Input, FT: Flow-Through, B: Bound

    Halo-trap Magnetic Agarose for immunoprecipitation of Halo-tag proteins. HEK293T cell lysate with Halo-tag protein. I: Input, FT: Flow-Through, B: Bound

  • Chromatin Immunoprecipitation (ChIP) utilizing Halo-Trap Magnetic Agarose (otma) was performed on cross-linked chromatin isolated from the liver of a transgenic mouse line expressing a Halo-tagged version of clock factor REVERBα (HaloReverbα). Samples were isolated at timepoints of non-peak (ZT20) or peak (ZT8) binding of the REVERBα protein to the Bmal1 promoter region of the genome. The enriched DNA was then quantified by ddPCR utilizing primers directed at a gene desert (negative control region) or the Bmal1 promoter. Fold enrichment of each sample DNA is relative to that of the negative control region of each sample.

    Chromatin Immunoprecipitation (ChIP) utilizing Halo-Trap Magnetic Agarose (otma) was performed on cross-linked chromatin isolated from the liver of a transgenic mouse line expressing a Halo-tagged version of clock factor REVERBα (HaloReverbα). Samples were isolated at timepoints of non-peak (ZT20) or peak (ZT8) binding of the REVERBα protein to the Bmal1 promoter region of the genome. The enriched DNA was then quantified by ddPCR utilizing primers directed at a gene desert (negative control region) or the Bmal1 promoter. Fold enrichment of each sample DNA is relative to that of the negative control region of each sample.

ChromoTek Halo-Trap Magnetic Agarose

The ChromoTek Halo-Trap Magnetic Agarose consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to magnetic agarose beads. Halo-Trap Magnetic Agarose is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.
Cat No. otma
Publications(3)

Specificity

Halo-Tag

Applications

IP, CoIP, ChIP, RIP

Type

Nanobody

Conjugate

Magnetic agarose

Synonyms

Halo-Trap, Halo, Halo tag

Size/Concentration: 

-/ -

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Product Information

The ChromoTek Halo-Trap Magnetic Agarose consists of an anti-Halo-tag Nanobody (VHH), which is covalently bound to magnetic agarose beads. Halo-Trap Magnetic Agarose is used to immunoprecipitate Halo-tag proteins from cell extracts of various organisms like mammals, plants, bacteria, yeast, insects etc. in the presence or absence of a covalently bound ligand. The interaction between Halo-Trap and the Halo-tag protein is reversible.

DescriptionImmunoprecipitation of Halo-tagged proteins and their interacting factors with anti-Halo Nanobody conjugated to magnetic agarose beads.

• Halo Trap recognizes Halo fusion proteins that are already bound to chloralkane-based ligands of the Halo-tag, e.g. dyes, biotin, etc.

• Halo-Trap can be used for affinity purification

• Bound Halo-fusion protein can be eluted without protease

• Structure and function are characterized

ApplicationsIP, CoIP, ChIP, RIP
Specificity/TargetHalo-tag (modified variant of the bacterial haloalkane dehalogenase enzyme from Rhodococcus rhodochrous) in the absence or presence of covalently bound chloralkane-based ligands.
Binding capacity12.5 μg of recombinant Halo-tag per 25 μL bead slurry
ConjugateMagnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
Elution bufferSDS sample buffer
0.2 M glycine pH 2.5
Wash buffer compatibility2 M urea, 2 M NaCl, 10 mM DTT,  2 % Nonidet P40 Substitute, 2 % Triton X-100
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 2 nM
Compatibility with mass spectrometryThe Halo-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2892995
Storage Buffer20% ethanol
Storage ConditionUpon receipt store at +4°C. Do not freeze!
Size25ul/reactions (eg：20rxns=500ul slurry)

Publications

ApplicationTitle
IP

Cell Rep

Increased degradation of FMRP contributes to neuronal hyperexcitability in tuberous sclerosis complex

Authors - Kellen D Winden
View Article
IP

Sci Adv

ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication

Authors - Guy J Pearson
View Article
IP

Immunity

Apolipoprotein E aggregation in microglia initiates Alzheimer's disease pathology by seeding β-amyloidosis

Authors - Seiji Kaji
View Article