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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPA14 Polyclonal antibody (28503-1-AP)

    WB analysis using 28503-1-AP (same clone as 28503-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28503-1-AP (HSPA14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28503-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using HSPA14 Polyclonal antibody (28503-1-AP)

    IHC staining of human ovary cancer using 28503-1-AP (same clone as 28503-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 28503-1-AP (HSPA14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28503-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using HSPA14 Polyclonal antibody (28503-1-AP)

    IF Staining of K-562 using 28503-1-AP (same clone as 28503-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HSPA14 antibody (28503-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28503-1-PBS in a different storage buffer formulation.

HSPA14 Polyclonal antibody, PBS Only

HSPA14 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 28503-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

HSP70 4, HSP60, Heat shock protein HSP60, Heat shock protein family A member 14, heat shock 70kDa protein 14

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28503-1-PBS targets HSPA14 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27240

Product name: Recombinant human HSPA14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-164 aa of BC119690

Sequence: MAAIGVHLGCTSACVAVYKDGRAGVVANDAGDRVTPAVVAYSENEEIVGLAAKQSRIRNISNTVMKVKQILGRSSSDPQAQKYIAESKCLVIEKNGKLRYEIDTGEETKFVNPEDVARLIFSKMKETAHSVLGSDANDVVITVPFDFGEKQKNALGEAARAAGF

相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 14
Observed molecular weight 55 kDa
GenBank accession numberBC119690
Gene Symbol HSPA14
Gene ID (NCBI) 51182
RRIDAB_3086059
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ0VDF9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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