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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HSPA14 Polyclonal antibody

HSPA14 Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 28503-1-AP

Synonyms

Heat shock 70 kDa protein 14, heat shock 70kDa protein 14, Heat shock protein HSP60, HSP60, HSP70 4, HSP70 like protein 1, HSP70L1, HSPA14

Validation Data Gallery

Applications:
  • WB analysis using 28503-1-AP

    WB analysis using 28503-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28503-1-AP (HSPA14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of K-562 using 28503-1-AP

    IF Staining of K-562 using 28503-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using HSPA14 antibody (28503-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IF detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

28503-1-AP targets HSPA14 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPA14 fusion protein Ag27240 相同性解析による交差性が予測される生物種
Full Name heat shock 70kDa protein 14
Observed molecular weight 55 kDa
GenBank accession numberBC119690
Gene symbol HSPA14
Gene ID (NCBI) 51182
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSPA14 antibody 28503-1-APDownload protocol
IF protocol for HSPA14 antibody 28503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols