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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPA12B Polyclonal antibody (19016-1-AP)

    WB analysis using 19016-1-AP (same clone as 19016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19016-1-AP (HSPA12B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19016-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using HSPA12B Polyclonal antibody (19016-1-AP)

    IP experiment of mouse brain using 19016-1-AP (same clone as 19016-1-PBS)

    IP result of anti-HSPA12B (IP:19016-1-AP, 4ug; Detection:19016-1-AP 1:800) with mouse brain tissue lysate 1840 ug. This data was developed using the same antibody clone with 19016-1-PBS in a different storage buffer formulation.

HSPA12B Polyclonal antibody, PBS Only

HSPA12B Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 19016-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

Heat shock protein family A member 12B, heat shock 70kD protein 12B, Heat shock 70 kDa protein 12B, C20orf60

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19016-1-PBS targets HSPA12B in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13544

Product name: Recombinant human HSPA12B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 341-686 aa of BC110881

Sequence: KELYKASGGPYGAVGVDLAFEQLLCRIFGEDFIATFKRQRPAAWVDLTIAFEARKRTAGPHRAGALNISLPFSFIDFYRKQRGHNVETALRRSSVNFVKWSSQGMLRMSCEAMNELFQPTVSGIIQHIEALLARPEVQGVKLLFLVGGFAESAVLQHAVQAALGARGLRVVVPHDVGLTILKGAVLFGQAPGVVRVRRSPLTYGVGVLNRFVPGRHPPEKLLVRDGRRWCTDVFERFVAAEQSVALGEEVRRSYCPARPGQRRVLINLYCCAAEDARFITDPGVRKCGALSLELEPADCGQDTAGAPPGRREIRAAMQFGDTEIKVTAVDVSTNRSVRASIDFLSN

相同性解析による交差性が予測される生物種
Full Name heat shock 70kD protein 12B
Calculated molecular weight 686 aa, 76 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC110881
Gene Symbol HSPA12B
Gene ID (NCBI) 116835
RRIDAB_3741957
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MM6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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