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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSPA12B Polyclonal antibody (19016-1-AP)

    WB analysis using 19016-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19016-1-AP (HSPA12B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using HSPA12B Polyclonal antibody (19016-1-AP)

    IP experiment of mouse brain using 19016-1-AP

    IP result of anti-HSPA12B (IP:19016-1-AP, 4ug; Detection:19016-1-AP 1:800) with mouse brain tissue lysate 1840 ug.

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HSPA12B Polyclonal antibody

HSPA12B Polyclonal Antibody for WB, IP, ELISA
Cat No. 19016-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

Heat shock protein family A member 12B, heat shock 70kD protein 12B, Heat shock 70 kDa protein 12B, C20orf60

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse heart tissue, mouse lung tissue, rat brain tissue
Positive IP detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19016-1-AP targets HSPA12B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSPA12B fusion protein Ag13544 相同性解析による交差性が予測される生物種
Full Name heat shock 70kD protein 12B
Calculated molecular weight 686 aa, 76 kDa
Observed molecular weight 75 kDa
GenBank accession numberBC110881
Gene Symbol HSPA12B
Gene ID (NCBI) 116835
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MM6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HSPA12B antibody 19016-1-APDownload protocol
IP protocol for HSPA12B antibody 19016-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols