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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP90AB1 Monoclonal antibody (67450-1-Ig)

    WB analysis using 67450-1-Ig (same clone as 67450-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67450-1-Ig (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67450-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using HSP90AB1 Monoclonal antibody (67450-1-Ig)

    WB analysis of HEK-293 using 67450-1-Ig (same clone as 67450-1-PBS)

    WB result of HSP90AB1 antibody (67450-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67450-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

HSP90AB1 Monoclonal antibody, PBS Only

HSP90AB1 Monoclonal Antibody for WB, ELISA
Cat No. 67450-1-PBS
Clone No.2D3D1

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Synonyms

D6S182, FLJ26984, Heat shock 84 kDa, Heat shock protein HSP 90 beta, HS90B, HSP 84, HSP 90, HSP84, HSP90 BETA, HSP90AB1, HSP90B, HSPC2, HSPCB

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67450-1-PBS targets HSP90AB1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HSP90AB1 fusion protein Ag28379 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Calculated molecular weight 723 aa, 83 kDa
Observed molecular weight 83-90 kDa
GenBank accession numberBC014485
Gene Symbol HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2882684
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08238
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers.