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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSP90AB1 Monoclonal antibody (67450-1-Ig)

    WB analysis using 67450-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67450-1-Ig (HSP90AB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using HSP90AB1 Monoclonal antibody (67450-1-Ig)

    WB analysis of HEK-293 using 67450-1-Ig

    WB result of HSP90AB1 antibody (67450-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP90AB1 transfected HEK-293 cells.

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  • Featured Product
  • KD/KO Validated

HSP90 Monoclonal antibody

HSP90 Monoclonal Antibody for WB, ELISA
Cat No. 67450-1-Ig
Clone No.2D3D1
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HSP90AB1, 2D3D1, Heat shock protein family C member 3, Heat shock protein HSP 90 beta, Heat shock protein HSP 90-beta

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, Neuro-2a cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67450-1-Ig targets HSP90 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen HSP90 fusion protein Ag28379 相同性解析による交差性が予測される生物種
Full Name heat shock protein 90kDa alpha (cytosolic), class B member 1
Calculated molecular weight 723 aa, 83 kDa
Observed molecular weight 83-90 kDa
GenBank accession numberBC014485
Gene Symbol HSP90AB1
Gene ID (NCBI) 3326
RRIDAB_2882684
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP08238
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HSP90, encoded by HSP90AB1, also known as HSP90 beta, is a member of HSP90 family which includes cytosolic HSP90 alpha (HSP90AA1) and HSP90 beta, endoplasmic reticulum related GRP74, mitochondrial TRAP1. HSP90 proteins play a versatile role in cell regulation, forming complexes with a large number of cellular kinases, transcription factors, and other molecules. Upregulation of HSP90AB1 has been found in various cancers. This antibody may cross-react with HSP90AA1 due to the high homology between them.

Protocols

Product Specific Protocols
WB protocol for HSP90 antibody 67450-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Virol Sin

Hsp90 β is Critical for the Infection of Severe Fever with Thrombocytopenia Syndrome Virus

Authors - Bo Wang
  • KD Validated
View Article