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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B12 Polyclonal antibody (27461-1-AP)

    WB analysis using 27461-1-AP (same clone as 27461-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27461-1-AP (HSD17B12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using HSD17B12 Polyclonal antibody (27461-1-AP)

    IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27461-1-AP (HSD17B12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using HSD17B12 Polyclonal antibody (27461-1-AP)

    IHC staining of mouse ovary using 27461-1-AP (same clone as 27461-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 27461-1-AP (HSD17B12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSD17B12 Polyclonal antibody (27461-1-AP)

    IF Staining of HepG2 using 27461-1-AP (same clone as 27461-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSD17B12 antibody (27461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27461-1-PBS in a different storage buffer formulation.

HSD17B12 Polyclonal antibody, PBS Only

HSD17B12 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 27461-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

17 beta HSD 12, 17-beta-HSD 12, 17-beta-hydroxysteroid dehydrogenase 12, 3 ketoacyl CoA reductase, 3-ketoacyl-CoA reductase

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27461-1-PBS targets HSD17B12 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26540

Product name: Recombinant human HSD17B12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 203-271 aa of BC012043

Sequence: SATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNG

相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 12
Calculated molecular weight 312 aa, 34 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012043
Gene Symbol HSD17B12
Gene ID (NCBI) 51144
RRIDAB_3669604
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GQ0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Hydroxysteroid 17-beta dehydrogenase 12 (HSD17B12, also known as KAR and SDR12C1) is a key enzyme of steroid metabolism pathway19 and is the human homolog of the yeast 3-ketoacyl-CoA reductase that catalyzes the second reaction in each VLCFA elongation cycle (PMID: 16166196; 12482854). It also acts as a drug-metabolizing enzyme (PMID: 36724833). HSD17B12 deficiency in embryonic stem cells reduced the production of arachidonic acid, a precursor of prostaglandins (PMID: 20130115).

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