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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HSD17B12 Polyclonal antibody (27461-1-AP)

    WB analysis using 27461-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27461-1-AP (HSD17B12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HSD17B12 Polyclonal antibody (27461-1-AP)

    IF Staining of HepG2 using 27461-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HSD17B12 antibody (27461-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HSD17B12 Polyclonal antibody

HSD17B12 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27461-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:1.1.1.330, 3-ketoacyl-CoA reductase, 3 ketoacyl CoA reductase, 17-beta-hydroxysteroid dehydrogenase 12, 17-beta-HSD 12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inCaco-2 cells, HCT 116 cells, HEK-293 cells, HT-29 cells, U-251 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27461-1-AP targets HSD17B12 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HSD17B12 fusion protein Ag26540 相同性解析による交差性が予測される生物種
Full Name hydroxysteroid (17-beta) dehydrogenase 12
Calculated molecular weight 312 aa, 34 kDa
Observed molecular weight30 kDa
GenBank accession numberBC012043
Gene Symbol HSD17B12
Gene ID (NCBI) 51144
RRIDAB_3669604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53GQ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Hydroxysteroid 17-beta dehydrogenase 12 (HSD17B12, also known as KAR and SDR12C1) is a key enzyme of steroid metabolism pathway19 and is the human homolog of the yeast 3-ketoacyl-CoA reductase that catalyzes the second reaction in each VLCFA elongation cycle (PMID: 16166196; 12482854). It also acts as a drug-metabolizing enzyme (PMID: 36724833). HSD17B12 deficiency in embryonic stem cells reduced the production of arachidonic acid, a precursor of prostaglandins (PMID: 20130115).

Protocols

Product Specific Protocols
WB protocol for HSD17B12 antibody 27461-1-APDownload protocol
IF protocol for HSD17B12 antibody 27461-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols