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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using HS1BP3 Polyclonal antibody (12845-1-AP)

    WB analysis of HT-1080 using 12845-1-AP (same clone as 12845-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12845-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using HS1BP3 Polyclonal antibody (12845-1-AP)

    WB analysis of Jurkat using 12845-1-AP (same clone as 12845-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12845-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HS1BP3 Polyclonal antibody (12845-1-AP)

    WB analysis of HeLa using 12845-1-AP (same clone as 12845-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12845-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NIH/3T3 cells using HS1BP3 Polyclonal antibody (12845-1-AP)

    WB analysis of NIH/3T3 using 12845-1-AP (same clone as 12845-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HS1BP3 Polyclonal antibody (12845-1-AP)

    IHC staining of human lung cancer using 12845-1-AP (same clone as 12845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HS1BP3 Polyclonal antibody (12845-1-AP)

    IHC staining of human lung cancer using 12845-1-AP (same clone as 12845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12845-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HS1BP3 Polyclonal antibody (12845-1-AP)

    IHC staining of human lung cancer using 12845-1-AP (same clone as 12845-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 25x lens). This data was developed using the same antibody clone with 12845-1-PBS in a different storage buffer formulation.

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HS1BP3 Polyclonal antibody, PBS Only

HS1BP3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12845-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12845-1-PBS targets HS1BP3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HS1BP3 fusion protein Ag3880 相同性解析による交差性が予測される生物種
Full Name HCLS1 binding protein 3
Calculated molecular weight 392 aa, 43 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC027947
Gene Symbol HS1BP3
Gene ID (NCBI) 64342
RRIDAB_10642005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53T59
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.