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Validation Data Gallery

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  • Western Blot (WB) analysis of HT-1080 cells using HS1BP3 Polyclonal antibody (12845-1-AP)

    WB analysis of HT-1080 using 12845-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using HS1BP3 Polyclonal antibody (12845-1-AP)

    WB analysis of Jurkat using 12845-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using HS1BP3 Polyclonal antibody (12845-1-AP)

    WB analysis of NIH/3T3 using 12845-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HS1BP3 Polyclonal antibody (12845-1-AP)

    WB analysis of HeLa using 12845-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HS1BP3 Polyclonal antibody (12845-1-AP)

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HS1BP3 Polyclonal antibody (12845-1-AP)

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HS1BP3 Polyclonal antibody (12845-1-AP)

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 25x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HS1BP3 Polyclonal antibody

HS1BP3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12845-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ETM2, HCLS1 binding protein 3, HS1 binding protein 3, HS1 BP3, HS1BP3, HSP1BP 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12845-1-AP targets HS1BP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HS1BP3 fusion protein Ag3880 相同性解析による交差性が予測される生物種
Full Name HCLS1 binding protein 3
Calculated molecular weight 392 aa, 43 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC027947
Gene Symbol HS1BP3
Gene ID (NCBI) 64342
RRIDAB_10642005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ53T59
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HS1BP3 antibody 12845-1-APDownload protocol
IHC protocol for HS1BP3 antibody 12845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols