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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

HS1BP3 Polyclonal antibody

HS1BP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12845-1-AP

Synonyms

ETM2, HCLS1 binding protein 3, HS1 binding protein 3, HS1 BP3, HS1BP3, HSP1BP 3

Validation Data Gallery

Applications:
  • WB analysis of HT-1080 using 12845-1-AP

    WB analysis of HT-1080 using 12845-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 12845-1-AP

    WB analysis of Jurkat using 12845-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 12845-1-AP

    WB analysis of NIH/3T3 using 12845-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12845-1-AP

    WB analysis of HeLa using 12845-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12845-1-AP (HS1BP3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12845-1-AP

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung cancer using 12845-1-AP

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human lung cancer using 12845-1-AP

    IHC staining of human lung cancer using 12845-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12845-1-AP (HS1BP3 antibody) at dilution of 1:100 (under 25x lens).

Tested Applications

Positive WB detected inHT-1080 cells, HeLa cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12845-1-AP targets HS1BP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HS1BP3 fusion protein Ag3880 相同性解析による交差性が予測される生物種
Full Name HCLS1 binding protein 3
Calculated molecular weight 392 aa, 43 kDa
Observed molecular weight 55-58 kDa
GenBank accession numberBC027947
Gene symbol HS1BP3
Gene ID (NCBI) 64342
RRIDAB_10642005
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for HS1BP3 antibody 12845-1-APDownload protocol
IHC protocol for HS1BP3 antibody 12845-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols