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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using HRPT2, CDC73 Polyclonal antibody (12310-1-AP)

    WB analysis of HEK-293 using 12310-1-AP (same clone as 12310-1-PBS)

    WB result of HRPT2, CDC73 antibody (12310-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HEK-293 cells. This data was developed using the same antibody clone with 12310-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using HRPT2, CDC73 Polyclonal antibody (12310-1-AP)

    WB analysis using 12310-1-AP (same clone as 12310-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12310-1-AP (HRPT2, CDC73 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12310-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using HRPT2, CDC73 Polyclonal antibody (12310-1-AP)

    IP experiment of mouse testis using 12310-1-AP (same clone as 12310-1-PBS)

    IP result of anti-HRPT2, CDC73 (IP:12310-1-AP, 4ug; Detection:12310-1-AP 1:500) with mouse testis tissue lysate 4000 ug. This data was developed using the same antibody clone with 12310-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HRPT2, CDC73 Polyclonal antibody (12310-1-AP)

    IHC staining of human breast cancer using 12310-1-AP (same clone as 12310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12310-1-AP (HRPT2, CDC73 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12310-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using HRPT2, CDC73 Polyclonal antibody (12310-1-AP)

    IHC staining of human breast cancer using 12310-1-AP (same clone as 12310-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12310-1-AP (HRPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12310-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HRPT2, CDC73 Polyclonal antibody (12310-1-AP)

    IF Staining of HeLa using 12310-1-AP (same clone as 12310-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRPT2, CDC73 antibody (12310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12310-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HRPT2, CDC73 Polyclonal antibody, PBS Only

HRPT2, CDC73 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12310-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12310-1-PBS targets HRPT2, CDC73 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag2959

Product name: Recombinant human HRPT2; CDC73 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 109-409 aa of BC014351

Sequence: WRTRTTILQSTGKNFSKNIFAILQSVKAREEGRAPEQRPAPNAAPVDPTLRTKQPIPAAYNRYDQERFKGKEETEGFKIDTMGTYHGMTLKSVTEGASARKTQTPAAQPVPRPVSQARPPPNQKKGSRTPIIIIPAATTSLITMLNAKDLLQDLKFVPSDEKKKQGCQRENETLIQRRKDQMQPGGTAISVTVPYRVVDQPLKLMPQDWDRVVAVFVQGPAWQFKGWPWLLPDGSPVDIFAKIKAFHLKYDEVRLDPNVQKWDVTVLELSYHKRHLDRPVFLRFWETLDRYMVKHKSHLRF

相同性解析による交差性が予測される生物種
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated molecular weight 531 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC014351
Gene Symbol HRPT2/CDC73
Gene ID (NCBI) 79577
RRIDAB_2078388
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6P1J9
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).

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