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  • Featured Product
  • KD/KO Validated

HRPT2, CDC73 Polyclonal antibody

HRPT2, CDC73 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12310-1-AP

Synonyms

C1orf28, CDC73, HPT JT, HRPT2, Hyperparathyroidism 2 protein, Parafibromin

Validation Data Gallery

Applications:
  • WB analysis using 12310-1-AP

    WB analysis using 12310-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12310-1-AP (HRPT2, CDC73 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12310-1-AP

    WB analysis of HEK-293 using 12310-1-AP

    WB result of HRPT2, CDC73 antibody (12310-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HRPT2, CDC73 transfected HEK-293 cells.

  • IP experiment of mouse testis using 12310-1-AP

    IP experiment of mouse testis using 12310-1-AP

    IP result of anti-HRPT2, CDC73 (IP:12310-1-AP, 4ug; Detection:12310-1-AP 1:500) with mouse testis tissue lysate 4000 ug.

  • IHC staining of human breast cancer using 12310-1-AP

    IHC staining of human breast cancer using 12310-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12310-1-AP (HRPT2, CDC73 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC73 antibody included
  • IHC staining of human breast cancer using 12310-1-AP

    IHC staining of human breast cancer using 12310-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12310-1-AP (HRPT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDC73 antibody included
  • IF Staining of HeLa using 12310-1-AP

    IF Staining of HeLa using 12310-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HRPT2, CDC73 antibody (12310-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, HepG2 cells
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CDC73 Ready-To-Use IHC Kit. CDC73 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

12310-1-AP targets HRPT2, CDC73 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HRPT2, CDC73 fusion protein Ag2959 相同性解析による交差性が予測される生物種
Full Name cell division cycle 73, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Calculated molecular weight 531 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC014351
Gene symbol CDC73
Gene ID (NCBI) 79577
RRIDAB_2078388
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Parafibromin is a product of the hyperparathyroidism-jaw tumor syndrome gene HRPT2/CDC73, a putative tumor suppressor gene recently implicated in the autosomal dominant hyperparathyroidism-jaw tumor familial cancer syndrome, sporadic parathyroid cancer, and a minority of families with isolated hyperparathyroidism (PMID: 15580289). Defects in CDC73 are causes of hyperparathyroidism type 1 (HRPT1) and hyperparathyroidism type 2 (HRPT2) as well as parathyroid carcinoma (PRTC) (PMID: 12434154). Tumor suppressor parafibromin to the transcription elongation and RNA processing pathway as a PAF1 complex- and RNA polymerase II-bound protein (PMID: 15923622). Besides, parafibromin is also involved in post-transcriptional control pathways (PMID: 16116486). Recent report has revealed that pathogenic mutation, such as CDC73 gene mutation, is able to affect histone monoubiquitination (PMID: 22021426).

Protocols

Product Specific Protocols
WB protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IHC protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IF protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
IP protocol for HRPT2, CDC73 antibody 12310-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IP

Cell

Inherited blood cancer predisposition through altered transcription elongation

Authors - Jiawei Zhao
View Article