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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPF1 Polyclonal antibody (28456-1-AP)

    WB analysis using 28456-1-AP (same clone as 28456-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28456-1-AP (HPF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28456-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using HPF1 Polyclonal antibody (28456-1-AP)

    IF Staining of A431 using 28456-1-AP (same clone as 28456-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using HPF1 antibody (28456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28456-1-PBS in a different storage buffer formulation.

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HPF1 Polyclonal antibody, PBS Only

HPF1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28456-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28456-1-PBS targets HPF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPF1 fusion protein Ag29377 相同性解析による交差性が予測される生物種
Full Name chromosome 4 open reading frame 27
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC010367
Gene Symbol HPF1
Gene ID (NCBI) 54969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWY4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

HPF1 is also known as C4orf27 and belongs to the HPF1 family. HPF1 is a novel interacting component of PARP1 complex that plays an important role in PARP1-dependent ADP-ribosylation signaling in the DNA damage response and the maintenance of genome stability (PMID: 29549427). The calculated molecular weight of HPF1 is 39 kDa.