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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HPF1 Polyclonal antibody (28456-1-AP)

    WB analysis using 28456-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28456-1-AP (HPF1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using HPF1 Polyclonal antibody (28456-1-AP)

    IF Staining of A431 using 28456-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using HPF1 antibody (28456-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

HPF1 Polyclonal antibody

HPF1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28456-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Histone PARylation factor 1, C4orf27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MOLT-4 cells
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28456-1-AP targets HPF1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HPF1 fusion protein Ag29377 相同性解析による交差性が予測される生物種
Full Name chromosome 4 open reading frame 27
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight39 kDa
GenBank accession numberBC010367
Gene Symbol HPF1
Gene ID (NCBI) 54969
RRIDAB_3086053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWY4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HPF1 is also known as C4orf27 and belongs to the HPF1 family. HPF1 is a novel interacting component of PARP1 complex that plays an important role in PARP1-dependent ADP-ribosylation signaling in the DNA damage response and the maintenance of genome stability (PMID: 29549427). The calculated molecular weight of HPF1 is 39 kDa.

Protocols

Product Specific Protocols
WB protocol for HPF1 antibody 28456-1-APDownload protocol
IF protocol for HPF1 antibody 28456-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols