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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HP1BP3 Polyclonal antibody (24556-1-AP)

    WB analysis using 24556-1-AP (same clone as 24556-1-PBS)

    Mouse thymus lysates were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using HP1BP3 Polyclonal antibody (24556-1-AP)

    WB analysis of MCF-7 using 24556-1-AP (same clone as 24556-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using HP1BP3 Polyclonal antibody (24556-1-AP)

    WB analysis of mouse thymus using 24556-1-AP (same clone as 24556-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HP1BP3 Polyclonal antibody (24556-1-AP)

    WB analysis of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 24556-1-AP (HP1BP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using HP1BP3 Polyclonal antibody (24556-1-AP)

    IP experiment of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

    IP result of anti-HP1BP3 (IP:24556-1-AP, 4ug; Detection:24556-1-AP 1:300) with HeLa cells lysate 3200ug. This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using HP1BP3 Polyclonal antibody (24556-1-AP)

    IHC staining of human skin cancer using 24556-1-AP (same clone as 24556-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 24556-1-AP (HP1BP3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HP1BP3 Polyclonal antibody (24556-1-AP)

    IF Staining of HeLa using 24556-1-AP (same clone as 24556-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HP1BP3 antibody (24556-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24556-1-PBS in a different storage buffer formulation.

HP1BP3 Polyclonal antibody, PBS Only

HP1BP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 24556-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Protein HP1-BP74, Protein HP1 BP74, HP1 BP74, Heterochromatin protein 1-binding protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24556-1-PBS targets HP1BP3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18709

Product name: Recombinant human HP1BP3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-302 aa of BC045660

Sequence: MASSPRPKMDAILTEAIKACFQKSGASVVAIRKYIIHKYPSLELERRGYLLKQALKRELNRGVIKQVKGKGASGSFVVVQKSRKTPQKSRNRKNRSSAVDPEPQVKLEDVLPLAFTRLCEPKEASYSLIRKYVSQYYPKLRVDIRPQLLKNALQRAVERGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEYAILSAIAAMNEPKTCSTTALKKYVLENHPGTNSNYQMHLLKKTLQKCEKNGWMEQISGKGFSGTFQLCFPYYPSPGVLFPKKEPDDSRDEDEDEDESSEEDSEDEEPPPK

相同性解析による交差性が予測される生物種
Full Name heterochromatin protein 1, binding protein 3
Calculated molecular weight 553 aa, 61 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC045660
Gene Symbol HP1BP3
Gene ID (NCBI) 50809
RRIDAB_2879606
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5SSJ5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Heterochromatin protein 1 binding protein 3(HP1BP3) associates with chromatin throughout the cell cycle and is required to maintain higher order chromatin structure during metaphase. HP1BP3 as a novel modulator of cognitive aging using a genetically diverse population of mice and confirm that HP1BP3 protein levels are significantly reduced in the hippocampi of cognitively impaired elderly humans relative to cognitively intact controls. Double-band staining is typical of HP1BP3 expression, indicative of multiple splice variants present in brain tissue.

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