Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse thymus tissue, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
24556-1-AP targets HP1BP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18709 Product name: Recombinant human HP1BP3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-302 aa of BC045660 Sequence: MASSPRPKMDAILTEAIKACFQKSGASVVAIRKYIIHKYPSLELERRGYLLKQALKRELNRGVIKQVKGKGASGSFVVVQKSRKTPQKSRNRKNRSSAVDPEPQVKLEDVLPLAFTRLCEPKEASYSLIRKYVSQYYPKLRVDIRPQLLKNALQRAVERGQLEQITGKGASGTFQLKKSGEKPLLGGSLMEYAILSAIAAMNEPKTCSTTALKKYVLENHPGTNSNYQMHLLKKTLQKCEKNGWMEQISGKGFSGTFQLCFPYYPSPGVLFPKKEPDDSRDEDEDEDESSEEDSEDEEPPPK 相同性解析による交差性が予測される生物種 |
| Full Name | heterochromatin protein 1, binding protein 3 |
| Calculated molecular weight | 553 aa, 61 kDa |
| Observed molecular weight | 70-75 kDa |
| GenBank accession number | BC045660 |
| Gene Symbol | HP1BP3 |
| Gene ID (NCBI) | 50809 |
| RRID | AB_2879606 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5SSJ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Heterochromatin protein 1 binding protein 3(HP1BP3) associates with chromatin throughout the cell cycle and is required to maintain higher order chromatin structure during metaphase. HP1BP3 as a novel modulator of cognitive aging using a genetically diverse population of mice and confirm that HP1BP3 protein levels are significantly reduced in the hippocampi of cognitively impaired elderly humans relative to cognitively intact controls. Double-band staining is typical of HP1BP3 expression, indicative of multiple splice variants present in brain tissue.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for HP1BP3 antibody 24556-1-AP | Download protocol |
| IHC protocol for HP1BP3 antibody 24556-1-AP | Download protocol |
| IF protocol for HP1BP3 antibody 24556-1-AP | Download protocol |
| IP protocol for HP1BP3 antibody 24556-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neurobiol Aging Systems genetics identifies Hp1bp3 as a novel modulator of cognitive aging. | ||
Sci Adv Cpeb1 remodels cell type-specific translational program to promote fear extinction |