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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOXA5 Polyclonal antibody (27622-1-AP)

    WB analysis using 27622-1-AP (same clone as 27622-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27622-1-AP (HOXA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27622-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HOXA5 Polyclonal antibody (27622-1-AP)

    IP experiment of HEK-293 using 27622-1-AP (same clone as 27622-1-PBS)

    IP result of anti-HOXA5 (IP:27622-1-AP, 4ug; Detection:27622-1-AP 1:500) with HEK-293 cells lysate 1360 ug. This data was developed using the same antibody clone with 27622-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using HOXA5 Polyclonal antibody (27622-1-AP)

    IF Staining of U2OS using 27622-1-AP (same clone as 27622-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using HOXA5 antibody (27622-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 27622-1-PBS in a different storage buffer formulation.

HOXA5 Polyclonal antibody, PBS Only

HOXA5 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 27622-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

homeobox A5, Homeobox protein Hox 1C, Homeobox protein Hox A5, Homeobox protein Hox-1C, Homeobox protein Hox-A5

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27622-1-PBS targets HOXA5 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26362

Product name: Recombinant human HOXA5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 64-163 aa of BC013682

Sequence: FGSGERARSYAASASAAPAEPRYSQPATSTHSPQPDPLPCSAVAPSPGSDSHHGGKNSLSNSSGASADAGSTHISSREGVGTASGAEEDAPASSEQASAQ

相同性解析による交差性が予測される生物種
Full Name homeobox A5
Calculated molecular weight 270 aa, 29 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC013682
Gene Symbol HOXA5
Gene ID (NCBI) 3202
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP20719
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HOXA5 (Homeobox A5) is a member of the homeobox gene family, which plays a crucial role in embryonic development, cell differentiation, and tissue patterning (PMID: 31159758). These genes encode transcription factors containing a conserved homeodomain that binds DNA to regulate the expression of target genes involved in morphogenesis and organogenesis.

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