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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HOXA5 Polyclonal antibody (27622-1-AP)

    WB analysis using 27622-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27622-1-AP (HOXA5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using HOXA5 Polyclonal antibody (27622-1-AP)

    IP experiment of HEK-293 using 27622-1-AP

    IP result of anti-HOXA5 (IP:27622-1-AP, 4ug; Detection:27622-1-AP 1:500) with HEK-293 cells lysate 1360 ug.

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HOXA5 Polyclonal antibody

HOXA5 Polyclonal Antibody for WB, IP, ELISA
Cat No. 27622-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Synonyms

homeobox A5, Homeobox protein Hox 1C, Homeobox protein Hox A5, Homeobox protein Hox-1C, Homeobox protein Hox-A5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA549 cells, HEK-293 cells
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27622-1-AP targets HOXA5 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HOXA5 fusion protein Ag26362 相同性解析による交差性が予測される生物種
Full Name homeobox A5
Calculated molecular weight 270 aa, 29 kDa
Observed molecular weight 38-42 kDa
GenBank accession numberBC013682
Gene Symbol HOXA5
Gene ID (NCBI) 3202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20719
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

HOXA5 (Homeobox A5) is a member of the homeobox gene family, which plays a crucial role in embryonic development, cell differentiation, and tissue patterning (PMID: 31159758). These genes encode transcription factors containing a conserved homeodomain that binds DNA to regulate the expression of target genes involved in morphogenesis and organogenesis.

Protocols

Product Specific Protocols
WB protocol for HOXA5 antibody 27622-1-APDownload protocol
IP protocol for HOXA5 antibody 27622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols