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Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using HLA-G Monoclonal antibody (66447-1-Ig)

    WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using HLA-G Monoclonal antibody (66447-1-Ig)

    WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human placenta tissue using HLA-G Monoclonal antibody (66447-1-Ig)

    WB analysis of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    Human placenta tissue and A375 cell lines (negtive control) were subjected to SDS PAGE followed by western blot with 66447-1-Ig (HLA-G Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HLA-G Monoclonal antibody (66447-1-Ig)

    IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using HLA-G Monoclonal antibody (66447-1-Ig)

    IHC staining of human placenta using 66447-1-Ig (same clone as 66447-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66447-1-Ig (HLA-G Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66447-1-PBS in a different storage buffer formulation.

HLA-G Monoclonal antibody, PBS Only

HLA-G Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66447-1-PBS
Clone No.1E5A10

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

HLA 6.0, HLA G, HLA G antigen, HLAG, HLA-G, MHC class I antigen G, MHC G

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66447-1-PBS targets HLA-G in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag10839

Product name: Recombinant human HLA-G protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 26-308 aa of BC021708

Sequence: SHSMRYFSAAVSRPSRGEPRFIAMGYVDDTQFVRFDSDSACPRMEPRAPWVEREGPEYWEEETRNTKAHAQTDRMNLQTLRGYYNQSEASSHTLQWMIGCDLGSDGRLLRGYEQYAYDGKDYLALNEDLRSWTAADTAAQISKRKCEAANVAEQRRAYLEGTCVEWLHRYLENGKEMLQRADPPKTHVTHHPVFDYEATLRCWALGFYPAEIILTWQRDGEDQTQDVELVETKPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLMLRWKQSSLPTIPI

相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, G
Calculated molecular weight 338 aa, 38 kDa
Observed molecular weight 33-45 kDa
GenBank accession numberBC021708
Gene Symbol HLA-G
Gene ID (NCBI) 3135
RRIDAB_2881816
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP17693
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). HLA-G is a non-classical MHC class I molecule with multiple immunoregulatory properties. HLA-G exhibits a restricted pattern of expression that includes placental extravillous trophoblasts at the maternal-fetal interface, where it abolishes maternal immune cell activity against fetus and establishes immune tolerance. Aberrant expression of HLA-G has been found in a variety of human neoplastic diseases. It plays an important role in the escape of tumor cells from immunosurveillance.