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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated HLA-F Monoclonal antibody

HLA-F Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B12C2

Cat no : CL488-66819

Synonyms

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

Validation Data Gallery

Applications:
  • FC experiment of Raji using CL488-66819

    FC experiment of Raji using CL488-66819

    1x10^6 Raji cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human HLA-F (CL488-66819, Clone: 1B12C2) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inRaji cells
For other applications, we recommend the unconjugated version of this antibody, 66819-1-Ig

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-66819 targets HLA-F in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen HLA-F fusion protein Ag6475 相同性解析による交差性が予測される生物種
Full Name major histocompatibility complex, class I, F
Calculated molecular weight 39 kDa
Observed molecular weight 40-45 kDa
GenBank accession numberBC062991
Gene symbol HLA-F
Gene ID (NCBI) 3134
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 HLA-F antibody CL488-66819Download protocol
Standard Protocols
Click here to view our Standard Protocols