Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 22231-1-AP (same clone as 22231-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
WB analysis of PC-3 using 22231-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 22231-1-AP (same clone as 22231-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
WB analysis of A549 using 22231-1-AP (same clone as 22231-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 22231-1-AP (same clone as 22231-1-PBS)
IP result of anti-HIP1 (IP:22231-1-AP, 4ug; Detection:22231-1-AP 1:1000) with HeLa cells lysate 2500ug. This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 22231-1-AP (same clone as 22231-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.
IF Staining of A549 using 22231-1-AP (same clone as 22231-1-PBS)
Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIP1 antibody (22231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 22231-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

HIP1 Polyclonal antibody, PBS Only

HIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 22231-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22231-1-PBS targets HIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag17576 Product name: Recombinant human HIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 435-764 aa of BC110545 Sequence: AELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIG 相同性解析による交差性が予測される生物種
Full Name	huntingtin interacting protein 1
Calculated molecular weight	1037 aa, 116 kDa
Observed molecular weight	116 kDa
GenBank accession number	BC110545
Gene Symbol	HIP1
Gene ID (NCBI)	3092
RRID	AB_2879041
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	O00291
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 22231-1-AP (same clone as 22231-1-PBS)

WB analysis of PC-3 using 22231-1-AP

WB analysis of human brain using 22231-1-AP (same clone as 22231-1-PBS)

WB analysis of A549 using 22231-1-AP (same clone as 22231-1-PBS)

IP experiment of HeLa using 22231-1-AP (same clone as 22231-1-PBS)

IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

IHC staining of human colon cancer using 22231-1-AP (same clone as 22231-1-PBS)

IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

IHC staining of human prostate cancer using 22231-1-AP (same clone as 22231-1-PBS)

IHC staining of mouse brain using 22231-1-AP (same clone as 22231-1-PBS)

IHC staining of human brain using 22231-1-AP (same clone as 22231-1-PBS)