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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIP1 Polyclonal antibody (22231-1-AP)

    WB analysis using 22231-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using HIP1 Polyclonal antibody (22231-1-AP)

    WB analysis of human brain using 22231-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using HIP1 Polyclonal antibody (22231-1-AP)

    WB analysis of A549 using 22231-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 22231-1-AP (HIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using HIP1 Polyclonal antibody (22231-1-AP)

    IP experiment of HeLa using 22231-1-AP

    IP result of anti-HIP1 (IP:22231-1-AP, 4ug; Detection:22231-1-AP 1:1000) with HeLa cells lysate 2500ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human colon cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human colon cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human prostate cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human prostate cancer using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of mouse brain using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22231-1-AP (HIP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human brain using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using HIP1 Polyclonal antibody (22231-1-AP)

    IHC staining of human brain using 22231-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 22231-1-AP (HIP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using HIP1 Polyclonal antibody (22231-1-AP)

    IF Staining of A549 using 22231-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using HIP1 antibody (22231-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Featured Product
  • KD/KO Validated

HIP1 Polyclonal antibody

HIP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 22231-1-AP
Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDU 145 cells, PC-3 cells, human brain tissue, A549 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human brain tissue, human colon cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22231-1-AP targets HIP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag17576

Product name: Recombinant human HIP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 435-764 aa of BC110545

Sequence: AELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIG

相同性解析による交差性が予測される生物種
Full Name huntingtin interacting protein 1
Calculated molecular weight 1037 aa, 116 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC110545
Gene Symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_2879041
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDO00291
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for HIP1 antibody 22231-1-APDownload protocol
IHC protocol for HIP1 antibody 22231-1-APDownload protocol
IP protocol for HIP1 antibody 22231-1-APDownload protocol
WB protocol for HIP1 antibody 22231-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Ecotoxicol Environ Saf

PFOA exposure decreases acrosome reaction and increases DNA oxidative damage by impairing the sperm head shaping in mice.

Authors - Chuan Wang
View Article
humanWB

Acta Biochim Biophys Sin (Shanghai)

Y-box protein-1 modulates circSPECC1 to promote glioma tumorigenesis via miR-615-5p/HIP1/AKT axis

Authors - Ping Lan
View Article
humanWB

FASEB J

MicroRNA-135a-3p regulates angiogenesis and tissue repair by targeting p38 signaling in endothelial cells.

Authors - Basak Icli
  • KD Validated
View Article

Life Sci Alliance

K48- and K63-linked ubiquitin chain interactome reveals branch- and length-specific ubiquitin interactors

Authors - Anita Waltho
View Article
humanWB,IHC

Oncol Lett

Abnormally high HIP1 expression is associated with metastatic behaviors and poor prognosis in ESCC.

Authors - Ying Sun
View Article
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