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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HIGD1A Polyclonal antibody (21749-1-AP)

    WB analysis using 21749-1-AP (same clone as 21749-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HIGD1A Polyclonal antibody (21749-1-AP)

    WB analysis of HeLa using 21749-1-AP (same clone as 21749-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21749-1-AP (HIGD1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using HIGD1A Polyclonal antibody (21749-1-AP)

    IHC staining of mouse ovary using 21749-1-AP (same clone as 21749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21749-1-AP (HIGD1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse ovary tissue using HIGD1A Polyclonal antibody (21749-1-AP)

    IHC staining of mouse ovary using 21749-1-AP (same clone as 21749-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 21749-1-AP (HIGD1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using HIGD1A Polyclonal antibody (21749-1-AP)

    IF Staining of HepG2 using 21749-1-AP (same clone as 21749-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HIGD1A antibody (21749-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A2780 cells using HIGD1A Polyclonal antibody (21749-1-AP)

    FC experiment of A2780 using 21749-1-AP (same clone as 21749-1-PBS)

    1x10^6 A2780 cells were intracellularly stained with 0.25 ug HIGD1A Polyclonal antibody (21749-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 21749-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HIGD1A Polyclonal antibody, PBS Only

HIGD1A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 21749-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HIG1, HIG1 domain family member 1A, HIG1 domain family member 1A, mitochondrial, HSPC010, Hypoxia-inducible gene 1 protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21749-1-PBS targets HIGD1A in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14027

Product name: Recombinant human HIGD1A protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-93 aa of BC000601

Sequence: MSTDTGVSLPSYEEDQGSKLIRKAKEAPFVPVGIAGFAAIVAYGLYKLKSRGNTKMSIHLIHMRVAAQGFVVGAMTVGMGYSMYREFWAKPKP

相同性解析による交差性が予測される生物種
Full Name HIG1 hypoxia inducible domain family, member 1A
Calculated molecular weight 93 aa, 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC000601
Gene Symbol HIGD1A
Gene ID (NCBI) 25994
RRIDAB_10858789
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y241
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

HIG1 domain family member 1A (HIGD1A) is a proposed subunit of cytochrome c oxidase (COX, complex IV), which is the terminal component of the mitochondrial respiratory chain that catalyzes the reduction of oxygen to water. May play a role in the assembly of respiratory supercomplexes (22342701). It is induced by hypoxia induction.This antibody is a rabbit polyclonal antibody raised against a full-length human HIGD1A protein.

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