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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using HERPUD1 Recombinant antibody (85627-1-RR)

    WB analysis of BxPC-3 using 85627-1-RR

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using HERPUD1 Recombinant antibody (85627-1-RR)

    WB analysis of HeLa using 85627-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using HERPUD1 Recombinant antibody (85627-1-RR)

    WB analysis of LNCaP using 85627-1-RR

    LNCaP cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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HERPUD1 Recombinant antibody

HERPUD1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85627-1-RR
Clone No.243054E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

HERP, Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, KIAA0025, Methyl methanesulfonate, MIF1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inLNCaP cells, BxPC-3 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85627-1-RR targets HERPUD1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen HERPUD1 fusion protein Ag34891 相同性解析による交差性が予測される生物種
Full Name homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
Calculated molecular weight 44 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC008320
Gene Symbol HERPUD1
Gene ID (NCBI) 9709
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).

Protocols

Product Specific Protocols
WB protocol for HERPUD1 antibody 85627-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols