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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using HERPUD1 Recombinant antibody (85627-1-RR)

    WB analysis of BxPC-3 using 85627-1-RR (same clone as 85627-1-PBS)

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using HERPUD1 Recombinant antibody (85627-1-RR)

    WB analysis of HeLa using 85627-1-RR (same clone as 85627-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of LNCaP cells using HERPUD1 Recombinant antibody (85627-1-RR)

    WB analysis of LNCaP using 85627-1-RR (same clone as 85627-1-PBS)

    LNCaP cells were subjected to SDS PAGE followed by western blot with 85627-1-RR (HERPUD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HERPUD1 Recombinant antibody (85627-1-RR)

    IHC staining of human lung cancer using 85627-1-RR (same clone as 85627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85627-1-RR (HERPUD1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HERPUD1 Recombinant antibody (85627-1-RR)

    IHC staining of human lung cancer using 85627-1-RR (same clone as 85627-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 85627-1-RR (HERPUD1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85627-1-PBS in a different storage buffer formulation.

HERPUD1 Recombinant monoclonal antibody, PBS Only

HERPUD1 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 85627-1-PBS
Clone No.243054E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

HERP, Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, KIAA0025, Methyl methanesulfonate, MIF1

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85627-1-PBS targets HERPUD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag34891

Product name: Recombinant human HERPUD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 94-391 aa of BC008320

Sequence: MPEINAKVAESTEEPAGSNRGQYPEDSSSDGLRQREVLRNLSSPGWENISRPEAAQQAFQGLGPGFSGYTPYGWLQLSWFQQIYARQYYMQYLAATAASGAFVPPPSAQEIPVVSAPAPAPIHNQFPAENQPANQNAAPQVVVNPGANQNLRMNAQGGPIVEEDDEINRDWLDWTYSAATFSVFLSILYFYSSLSRFLMVMGATVVMYLHHVGWFPFRPRPVQNFPNDGPPPDVVNQDPNNNLQEGTDPETEDPNHLPPDRDVLDGEQTSPSFMSTAWLVFKTFFASLLPEGPPAIAN*

相同性解析による交差性が予測される生物種
Full Name homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1
Calculated molecular weight 44 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC008320
Gene Symbol HERPUD1
Gene ID (NCBI) 9709
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ15011
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (HERPUD1), also called HERP or MIF1, is component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. HERP is strongly induced by ER stress but rapidly degraded by proteasome. HERP interacts with members of the ubiquilin family, which function as a shuttle factor to deliver ubiquitinated substrates to the proteasome for degradation. HERP is present in activated microglia in senile plaques in the brain of patients with Alzheimer disease, and there is evidence suggesting HERP increase nigro-striatal dopaminergic activity and is able to improve symptoms in patients with Parkinson's disease, levodopa-related dyskinesias and Tardive dyskinesia. MW of HERP is 54 kDa (PMID: 23620059).

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