Validation Data Gallery
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
Product Information
HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Amplification and overexpression of HER2 have been reported in numerous cancers, including breast and ovarian tumors. HER2 is a therapeutic target for the treatment of breast cancer and other carcinomas.
| Source | CHO cells-derived Human HER2/ErbB2 protein (23-652 aa) (Accession #P04626) with a mutated Human IgG1 FcZero backbone |
| Isotype | Recombinant Human IgG1 |
| Tested Reactivity | Human |
| Applications | FC |
| Target Cell | HER2/ErbB2-specific CAR-T cells, HER2/ErbB2-specific CAR-NK cells |
| Backbone | Human IgG1 FcZero backbone |
| Site Specific Conjugation | Site specific conjugated with Biotin |
| Form | Liquid |
| GeneID | 2064 |
| UNIPROT ID | P04626 |
| Conjugate | Biotin |
| Excitation/Emission Maxima Wavelengths | - |
| Storage Buffer | 50mM HEPES, 150mM NaCl, 5mM EDTA, 0.1% CHAPS, 10% Sucrose and 0.1% Proclin 300, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |