Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL750-82950 targets HEBP1 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag10538 Product name: Recombinant human HEBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-189 aa of BC016277 Sequence: MLGMIKNSLFGSVETWPWQVLSKGDKEEVAYEERACEGGKFATVEVTDKPVDEALREAMPKVAKYAGGTNDKGIGMGMTVPISFAVFPNEDGSLQKKLKVWFRIPNQFQSDPPAPSDKSVKIEEREGITVYSMQFGGYAKEADYVAQATRLRAALEGTATYRGDIYFCTGYDPPMKPYGRRNEIWLLKT 相同性解析による交差性が予測される生物種 |
| Full Name | heme binding protein 1 |
| Calculated molecular weight | 189 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC016277 |
| Gene Symbol | HEBP1 |
| Gene ID (NCBI) | 50865 |
| RRID | AB_3673751 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 755 nm / 780 nm |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NRV9 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 HEBP1 antibody CL750-82950 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |