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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using HDAC8 Polyclonal antibody (17548-1-AP)

    WB analysis using 17548-1-AP (same clone as 17548-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of Raji cells using HDAC8 Polyclonal antibody (17548-1-AP)

    IP experiment of Raji using 17548-1-AP (same clone as 17548-1-PBS)

    IP result of anti-HDAC8 (IP:17548-1-AP, 4ug; Detection:17548-1-AP 1:600) with Raji cells lysate 2800ug. This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HDAC8 Polyclonal antibody (17548-1-AP)

    IHC staining of human lung cancer using 17548-1-AP (same clone as 17548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using HDAC8 Polyclonal antibody (17548-1-AP)

    IHC staining of human lung cancer using 17548-1-AP (same clone as 17548-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17548-1-AP (HDAC8 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using HDAC8 Polyclonal antibody (17548-1-AP)

    IF Staining of HeLa using 17548-1-AP (same clone as 17548-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17548-1-AP (HDAC8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17548-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17548-1-PBS targets HDAC8 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HDAC8 fusion protein Ag11696 相同性解析による交差性が予測される生物種
Full Name histone deacetylase 8
Calculated molecular weight 377 aa, 42 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC050433
Gene Symbol HDAC8
Gene ID (NCBI) 55869
RRIDAB_2116926
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BY41
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. At least 4 classes of HDAC were identified. As a class I HDAC, HDAC 8 was primarily found in the nucleus. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. This antibody is a rabbit polyclonal antibody raised against full length HDAC8 of human origin.